A simple phenotypic method for screening of MCR-1-mediated colistin resistance
نویسندگان
چکیده
منابع مشابه
Multiplex PCR for detection of plasmid-mediated colistin resistance determinants, mcr-1, mcr-2, mcr-3, mcr-4 and mcr-5 for surveillance purposes
Background and aimPlasmid-mediated colistin resistance mechanisms have been identified worldwide in the past years. A multiplex polymerase chain reaction (PCR) protocol for detection of all currently known transferable colistin resistance genes (mcr-1 to mcr-5, and variants) in Enterobacteriaceae was developed for surveillance or research purposes. Methods: We designed four new primer pairs to ...
متن کاملDissemination and Mechanism for the MCR-1 Colistin Resistance
Polymyxins are the last line of defense against lethal infections caused by multidrug resistant Gram-negative pathogens. Very recently, the use of polymyxins has been greatly challenged by the emergence of the plasmid-borne mobile colistin resistance gene (mcr-1). However, the mechanistic aspects of the MCR-1 colistin resistance are still poorly understood. Here we report the comparative genomi...
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Antibiotic resistance is a prevalent problem in public health worldwide. In general, the carbapenem β-lactam antibiotics are considered a final resort against lethal infections by multidrug-resistant bacteria. Colistin is a cationic polypeptide antibiotic and acts as the last line of defense for treatment of carbapenem-resistant bacteria. Very recently, a new plasmid-borne colistin resistance g...
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Katrin Zurfluh, Nicolas Kieffer, Laurent Poirel, Patrice Nordmann, Roger Stephan Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, Switzerland; Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Fribourg, Switzerland; INSERM European Unit (LEA Paris, France), University of Fribourg, Fribourg, Swi...
متن کاملmcr-1 Colistin Resistance in ESBL-Producing Klebsiella pneumoniae, France
We report intestinal carriage of an extended-spectrum β-lactamase-producing Klebsiella pneumoniae strain with high-level resistance to colistin (MIC 24 mg/L) in a patient in France who had been hospitalized for fungal meningitis. The strain had the mcr-1 plasmid gene and an inactivated mgrB gene, which are associated with colistin resistance.
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ژورنال
عنوان ژورنال: Clinical Microbiology and Infection
سال: 2018
ISSN: 1198-743X
DOI: 10.1016/j.cmi.2017.08.011